原理介紹

無(wú)銅點(diǎn)擊化學(xué)反應(yīng)，即菌株促進(jìn)的疊氮炔環(huán)加成（SPAAC）是一種生物正交反應(yīng)，利用一對(duì)試劑，即環(huán)辛基和疊氮化合物，它們彼此有效地反應(yīng)，同時(shí)對(duì)羥基、氨基和硫醇等天然官能團(tuán)保持惰性。SPAAC能夠通過(guò)形成穩(wěn)定的三唑，在水和其他復(fù)雜的化學(xué)環(huán)境中標(biāo)記各種各樣的生物分子，而無(wú)需任何輔助試劑。在眾多已知的環(huán)辛基化合物中，二苯并環(huán)辛基化合物（DBCO）是一類(lèi)具有相當(dāng)快的動(dòng)力學(xué)和良好的水緩沖穩(wěn)定性的試劑。在生理溫度和pH范圍內(nèi)，DBCO基團(tuán)不會(huì)與許多生物分子中天然存在的巰基、氨基或羥基發(fā)生反應(yīng)。DBCO-PEG4-琥珀酰亞胺酯是一種常用的通過(guò)氨基修飾將DBCO基團(tuán)引入生物分子的試劑。靶點(diǎn)科技分銷(xiāo)的ClickChemistryTools的DBCO-PEG4-NHS Ester，是高效率的DBCO 琥珀酰亞胺酯。

化學(xué)結(jié)構(gòu)

參考文獻(xiàn)

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